mouse α-agps antibody  (Santa Cruz Biotechnology)

Journal: International Journal of Molecular Sciences

Article Title: Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome

doi: 10.3390/ijms18122750

Figure Lengend Snippet: Differentially expressed serum proteins identified by in-solution digestion and LC-MS/MS analysis in patients with primary Sjögren’s syndrome (pSS) and healthy controls (HCs).

Article Snippet: A1AG1 (2 μg of protein in 10% SDS-PAGE) or A1AT (2 μg of protein in 8% SDS-PAGE) was evaluated using a mouse anti-A1AG1 monoclonal antibody (sc-69753, Santa Cruz Biotechnology, Dallas, TX, USA) or a mouse anti-A1AT monoclonal antibody (sc-69752, Santa Cruz Biotechnology).

Techniques: Coagulation

Journal: International Journal of Molecular Sciences

Article Title: Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome

doi: 10.3390/ijms18122750

Figure Lengend Snippet: Protein levels of A1AG1 and A1AT in serum were examined using anti-A1AG1 ( A ) and anti-A1AT ( B ) antibodies through Western blotting. Average blot densitometric values were calculated from duplicate data. Percentages of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and loading amounts of serum proteins used in Western blotting were 10% and 2 μg for A1AG1, and 8% and 2 μg for A1AT, respectively. A duplicate gel was stained with Coomassie brilliant blue (CBB) as a loading control (right, bottom panel). The red arrow indicates the A1AG1 or A1AT protein. Receiver operating characteristic (ROC) curves were generated according to blot densitometry of A1AG1 and A1AT. The area under the ROC curve (AUC), sensitivity, and specificity were further estimated ( C ).

Article Snippet: A1AG1 (2 μg of protein in 10% SDS-PAGE) or A1AT (2 μg of protein in 8% SDS-PAGE) was evaluated using a mouse anti-A1AG1 monoclonal antibody (sc-69753, Santa Cruz Biotechnology, Dallas, TX, USA) or a mouse anti-A1AT monoclonal antibody (sc-69752, Santa Cruz Biotechnology).

Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Control, Generated